5-AMINO-1MQ - My Framer Site

Overview

5-Amino-1MQ is a small molecule amine compound designed as an inhibitor of nicotinamide N-methyltransferase (NNMT) enzyme. The compound blocks NAD+ depletion and enhances cellular NAD+ availability, supporting metabolic health, energy production, and mitochondrial function. 5-Amino-1MQ is extensively studied for its potential in supporting metabolic flexibility, energy expenditure, and body composition improvement. Research indicates the compound inhibits NNMT-mediated NAD+ depletion, enhances NAD+-dependent sirtuin signaling, and promotes metabolic adaptations similar to caloric restriction and exercise. 5-Amino-1MQ represents a valuable research tool for investigating NAD+ metabolism, NNMT inhibition, metabolic regulation, and therapeutic approaches to metabolic dysfunction and obesity.

Overview

5-Amino-1MQ is a small molecule amine compound designed as an inhibitor of nicotinamide N-methyltransferase (NNMT) enzyme. The compound blocks NAD+ depletion and enhances cellular NAD+ availability, supporting metabolic health, energy production, and mitochondrial function. 5-Amino-1MQ is extensively studied for its potential in supporting metabolic flexibility, energy expenditure, and body composition improvement. Research indicates the compound inhibits NNMT-mediated NAD+ depletion, enhances NAD+-dependent sirtuin signaling, and promotes metabolic adaptations similar to caloric restriction and exercise. 5-Amino-1MQ represents a valuable research tool for investigating NAD+ metabolism, NNMT inhibition, metabolic regulation, and therapeutic approaches to metabolic dysfunction and obesity.

Overview

5-Amino-1MQ is a small molecule amine compound designed as an inhibitor of nicotinamide N-methyltransferase (NNMT) enzyme. The compound blocks NAD+ depletion and enhances cellular NAD+ availability, supporting metabolic health, energy production, and mitochondrial function. 5-Amino-1MQ is extensively studied for its potential in supporting metabolic flexibility, energy expenditure, and body composition improvement. Research indicates the compound inhibits NNMT-mediated NAD+ depletion, enhances NAD+-dependent sirtuin signaling, and promotes metabolic adaptations similar to caloric restriction and exercise. 5-Amino-1MQ represents a valuable research tool for investigating NAD+ metabolism, NNMT inhibition, metabolic regulation, and therapeutic approaches to metabolic dysfunction and obesity.

Biochemical Characteristics

5-Amino-1MQ is a small molecule compound with molecular weight approximately 170 Da. The compound is a selective NNMT inhibitor designed to block the enzymatic conversion of nicotinamide to N-methylnicotinamide (MNA), preventing NAD+ depletion. The compound is water-soluble and demonstrates stability across physiological pH ranges (6.5–8.0). 5-Amino-1MQ exhibits excellent cell membrane penetration and bioavailability, efficiently distributing to tissues involved in metabolic regulation. Cellular uptake occurs through diffusion and transporter-mediated mechanisms. The compound localizes to cells expressing NNMT enzyme including adipocytes, hepatocytes, and muscle cells where metabolic effects are exerted.

Biochemical Characteristics

5-Amino-1MQ is a small molecule compound with molecular weight approximately 170 Da. The compound is a selective NNMT inhibitor designed to block the enzymatic conversion of nicotinamide to N-methylnicotinamide (MNA), preventing NAD+ depletion. The compound is water-soluble and demonstrates stability across physiological pH ranges (6.5–8.0). 5-Amino-1MQ exhibits excellent cell membrane penetration and bioavailability, efficiently distributing to tissues involved in metabolic regulation. Cellular uptake occurs through diffusion and transporter-mediated mechanisms. The compound localizes to cells expressing NNMT enzyme including adipocytes, hepatocytes, and muscle cells where metabolic effects are exerted.

Biochemical Characteristics

5-Amino-1MQ is a small molecule compound with molecular weight approximately 170 Da. The compound is a selective NNMT inhibitor designed to block the enzymatic conversion of nicotinamide to N-methylnicotinamide (MNA), preventing NAD+ depletion. The compound is water-soluble and demonstrates stability across physiological pH ranges (6.5–8.0). 5-Amino-1MQ exhibits excellent cell membrane penetration and bioavailability, efficiently distributing to tissues involved in metabolic regulation. Cellular uptake occurs through diffusion and transporter-mediated mechanisms. The compound localizes to cells expressing NNMT enzyme including adipocytes, hepatocytes, and muscle cells where metabolic effects are exerted.

Research Applications

NAD+ Enhancement Investigation of 5-Amino-1MQ-induced elevation of cellular NAD+ levels through NNMT inhibition. Metabolic Rate Enhancement Assessment of increased energy expenditure and metabolic rate through NAD+-dependent mechanisms. Body Weight Reduction Study of body weight loss and improved body composition through enhanced metabolic flexibility. Adipose Tissue Remodeling Investigation of browning of white adipose tissue and enhanced brown adipose tissue thermogenesis. Glucose Homeostasis Assessment of improvements in glucose tolerance and insulin sensitivity through metabolic enhancement. Mitochondrial Function Study of enhancement of mitochondrial oxidative capacity and ATP production efficiency. Sirtuin Activation Investigation of NAD+-dependent sirtuin pathway enhancement and activation of longevity pathways.

Research Applications

NAD+ Enhancement Investigation of 5-Amino-1MQ-induced elevation of cellular NAD+ levels through NNMT inhibition. Metabolic Rate Enhancement Assessment of increased energy expenditure and metabolic rate through NAD+-dependent mechanisms. Body Weight Reduction Study of body weight loss and improved body composition through enhanced metabolic flexibility. Adipose Tissue Remodeling Investigation of browning of white adipose tissue and enhanced brown adipose tissue thermogenesis. Glucose Homeostasis Assessment of improvements in glucose tolerance and insulin sensitivity through metabolic enhancement. Mitochondrial Function Study of enhancement of mitochondrial oxidative capacity and ATP production efficiency. Sirtuin Activation Investigation of NAD+-dependent sirtuin pathway enhancement and activation of longevity pathways.

Research Applications

NAD+ Enhancement Investigation of 5-Amino-1MQ-induced elevation of cellular NAD+ levels through NNMT inhibition. Metabolic Rate Enhancement Assessment of increased energy expenditure and metabolic rate through NAD+-dependent mechanisms. Body Weight Reduction Study of body weight loss and improved body composition through enhanced metabolic flexibility. Adipose Tissue Remodeling Investigation of browning of white adipose tissue and enhanced brown adipose tissue thermogenesis. Glucose Homeostasis Assessment of improvements in glucose tolerance and insulin sensitivity through metabolic enhancement. Mitochondrial Function Study of enhancement of mitochondrial oxidative capacity and ATP production efficiency. Sirtuin Activation Investigation of NAD+-dependent sirtuin pathway enhancement and activation of longevity pathways.

Pathway / Mechanistic Context

NNMT Inhibition 5-Amino-1MQ selectively inhibits nicotinamide N-methyltransferase (NNMT) enzyme, blocking the conversion of nicotinamide to N-methylnicotinamide (MNA) and preventing NAD+ depletion. NAD+ Elevation By inhibiting NNMT-mediated NAD+ consumption, 5-Amino-1MQ increases cellular NAD+ levels, providing enhanced substrate availability for NAD+-dependent enzymes including sirtuins and PARPs. Sirtuin Activation Enhanced NAD+ availability promotes activation of NAD+-dependent sirtuins (SIRT1-7), which enhance mitochondrial biogenesis, metabolic flexibility, and cellular stress resistance. Brown Adipose Tissue Activation The compound promotes conversion of white adipocytes to brown/beige adipocytes through activation of brown adipose tissue thermogenic pathways, increasing energy expenditure. Mitochondrial Biogenesis 5-Amino-1MQ-induced NAD+ elevation activates PGC-1α through sirtuin-dependent mechanisms, promoting mitochondrial biogenesis and enhanced oxidative capacity. Metabolic Switching Enhancement The compound enhances metabolic flexibility and ability to switch between glucose and lipid oxidation through activation of AMPK and other metabolic regulators dependent on NAD+ availability.

Pathway / Mechanistic Context

NNMT Inhibition 5-Amino-1MQ selectively inhibits nicotinamide N-methyltransferase (NNMT) enzyme, blocking the conversion of nicotinamide to N-methylnicotinamide (MNA) and preventing NAD+ depletion. NAD+ Elevation By inhibiting NNMT-mediated NAD+ consumption, 5-Amino-1MQ increases cellular NAD+ levels, providing enhanced substrate availability for NAD+-dependent enzymes including sirtuins and PARPs. Sirtuin Activation Enhanced NAD+ availability promotes activation of NAD+-dependent sirtuins (SIRT1-7), which enhance mitochondrial biogenesis, metabolic flexibility, and cellular stress resistance. Brown Adipose Tissue Activation The compound promotes conversion of white adipocytes to brown/beige adipocytes through activation of brown adipose tissue thermogenic pathways, increasing energy expenditure. Mitochondrial Biogenesis 5-Amino-1MQ-induced NAD+ elevation activates PGC-1α through sirtuin-dependent mechanisms, promoting mitochondrial biogenesis and enhanced oxidative capacity. Metabolic Switching Enhancement The compound enhances metabolic flexibility and ability to switch between glucose and lipid oxidation through activation of AMPK and other metabolic regulators dependent on NAD+ availability.

Pathway / Mechanistic Context

NNMT Inhibition 5-Amino-1MQ selectively inhibits nicotinamide N-methyltransferase (NNMT) enzyme, blocking the conversion of nicotinamide to N-methylnicotinamide (MNA) and preventing NAD+ depletion. NAD+ Elevation By inhibiting NNMT-mediated NAD+ consumption, 5-Amino-1MQ increases cellular NAD+ levels, providing enhanced substrate availability for NAD+-dependent enzymes including sirtuins and PARPs. Sirtuin Activation Enhanced NAD+ availability promotes activation of NAD+-dependent sirtuins (SIRT1-7), which enhance mitochondrial biogenesis, metabolic flexibility, and cellular stress resistance. Brown Adipose Tissue Activation The compound promotes conversion of white adipocytes to brown/beige adipocytes through activation of brown adipose tissue thermogenic pathways, increasing energy expenditure. Mitochondrial Biogenesis 5-Amino-1MQ-induced NAD+ elevation activates PGC-1α through sirtuin-dependent mechanisms, promoting mitochondrial biogenesis and enhanced oxidative capacity. Metabolic Switching Enhancement The compound enhances metabolic flexibility and ability to switch between glucose and lipid oxidation through activation of AMPK and other metabolic regulators dependent on NAD+ availability.

Preclinical Research Summary

5-Amino-1MQ is supplied as pharmaceutical-grade powder at purities of ≥98%. Standard concentrations available in solution form at 5–50 mM. Storage at room temperature (15–30°C) in sealed containers; shelf-life typically 24–36 months. Analytical testing includes HPLC purity verification (≥98%), HPLC-MS molecular confirmation, elemental analysis, and chemical stability assessment. Bioassay verification of NNMT inhibitory activity available upon request. Certificate of Analysis provided with each lot.

Preclinical Research Summary

5-Amino-1MQ is supplied as pharmaceutical-grade powder at purities of ≥98%. Standard concentrations available in solution form at 5–50 mM. Storage at room temperature (15–30°C) in sealed containers; shelf-life typically 24–36 months. Analytical testing includes HPLC purity verification (≥98%), HPLC-MS molecular confirmation, elemental analysis, and chemical stability assessment. Bioassay verification of NNMT inhibitory activity available upon request. Certificate of Analysis provided with each lot.

Preclinical Research Summary

5-Amino-1MQ is supplied as pharmaceutical-grade powder at purities of ≥98%. Standard concentrations available in solution form at 5–50 mM. Storage at room temperature (15–30°C) in sealed containers; shelf-life typically 24–36 months. Analytical testing includes HPLC purity verification (≥98%), HPLC-MS molecular confirmation, elemental analysis, and chemical stability assessment. Bioassay verification of NNMT inhibitory activity available upon request. Certificate of Analysis provided with each lot.

RUO Disclaimer

RESEARCH USE ONLY (RUO) This product is intended exclusively for laboratory research and is not approved for clinical use or human/animal administration. Not manufactured for pharmaceutical use. Users assume responsibility for suitability determination, proper storage, safety compliance, and regulatory adherence. Institutional approval (IACUC, IRB) may be required. Information provided is for research reference only and does not constitute medical advice or therapeutic claims.

RUO Disclaimer

RESEARCH USE ONLY (RUO) This product is intended exclusively for laboratory research and is not approved for clinical use or human/animal administration. Not manufactured for pharmaceutical use. Users assume responsibility for suitability determination, proper storage, safety compliance, and regulatory adherence. Institutional approval (IACUC, IRB) may be required. Information provided is for research reference only and does not constitute medical advice or therapeutic claims.

RUO Disclaimer

RESEARCH USE ONLY (RUO) This product is intended exclusively for laboratory research and is not approved for clinical use or human/animal administration. Not manufactured for pharmaceutical use. Users assume responsibility for suitability determination, proper storage, safety compliance, and regulatory adherence. Institutional approval (IACUC, IRB) may be required. Information provided is for research reference only and does not constitute medical advice or therapeutic claims.